PAX6 ELISA Kit (Mouse) (OKCA00912)

Catalog No: OKCA00912

Size:96 Wells

Mouse PAX6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	99	94-103
  1:2	95	89-99
  1:4	101	97-105
  1:8	92	88-96

• ELISA Kit Principle: Aviva Systems Biology Pax6 ELISA Kit (Mouse) (OKCA00912) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Pax6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pax6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Pax6 captured in well.
• ELISA Kit Range: 46.88 pg/mL-3,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90	86-94
  EDTA Plasma (n=4)	98	93-102

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Pax6 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Pax6 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Pax6 Microplate: 96 Wells (12 x 8 Well strips)
  • Pax6 Lyophilized Standard: 2
  • 100X Biotinylated Pax6 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 11.72 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PAX6
• Gene Full Name: Paired box 6
• Alias Symbols: Dey, Pax, Sey, AEY1, AEY11, Pax-6, Gsfaey, Gsfaey11, 1500038E17Rik
• NCBI Gene Id: 18508
• Protein Name: Paired box protein Pax-6
• Description of Target: Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells. Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains.
• Uniprot ID: P63015
• Protein Accession #: NP_001231127.1
• Nucleotide Accession #: NM_001244198.2