PTPRJ ELISA Kit (Human) (OKCD01590)

Catalog No: OKCD01590 (Formerly GWB-KBBRT9)

Size:96WELLS

Human PTPRJ ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Density Enhanced Phosphatase 1 (DEP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Density Enhanced Phosphatase 1 (DEP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Density Enhanced Phosphatase 1 (DEP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Density Enhanced Phosphatase 1 (DEP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Density Enhanced Phosphatase 1 (DEP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Density Enhanced Phosphatase 1 (DEP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PTPRJ Microplate: 96 Wells (12 x 8 Well Strips)
  • PTPRJ Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PTPRJ Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Receptor Type J (PTPRJ). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTPRJ
• Gene Full Name: protein tyrosine phosphatase receptor type J
• Alias Symbols: CD148;CD148 antigen;density-enhanced phosphatase 1;DEP1;DEP-1;HPTP eta;HPTPeta;human density enhanced phosphatase-1;protein tyrosine phosphatase, receptor type, J polypeptide;protein-tyrosine phosphatase eta;Protein-tyrosine phosphatase receptor type J;receptor-type tyrosine-protein phosphatase eta;R-PTP-ETA;R-PTP-J;SCC1;susceptibility to colon cancer 1, mouse, homolog of.
• NCBI Gene Id: 5795
• Protein Name: Receptor-type tyrosine-protein phosphatase eta
• Description of Target: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
• Uniprot ID: Q12913
• Protein Accession #: NP_001091973.1
• Nucleotide Accession #: NM_001098503.1
• Protein Size (# AA): 1337