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Catalog No: OKCD01770 (Formerly GWB-KBBQQ3)
Size:96WELLS
Price: $675.00
SKU
OKCD01770
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Datasheets/ManualsPrintable datasheet for PTGR1 ELISA Kit (Human) (OKCD01770)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-101%86-98%81-104%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Reductase 1 (PTGR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Reductase 1 (PTGR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Reductase 1 (PTGR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prostaglandin Reductase 1 (PTGR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum90-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Reductase 1 (PTGR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Reductase 1 (PTGR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PTGR1 Microplate96 Wells (12 x 8 Well Strips)
PTGR1 Lyophilized Standard2 x 10 ng
100X Biotinylated PTGR1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Prostaglandin Reductase 1 (PTGR1). No significant cross-reactivity or interference between Prostaglandin Reductase 1 (PTGR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPTGR1
Gene Full Nameprostaglandin reductase 1
Alias Symbols15-oxoprostaglandin 13-reductase;D3T-inducible gene 1 protein;DIG-1;dithiolethione-inducible gene 1 protein;leukotriene B4 12-hydroxydehydrogenase;LTB4DH;NAD(P)H-dependent alkenal/one oxidoreductase;NADP-dependent leukotriene B4 12-hydroxydehydrogenase;PGR1;PRG-1;prostaglandin reductase 1;ZADH3;zinc binding alcohol dehydrogenase domain containing 3.
NCBI Gene Id22949
Protein NameProstaglandin reductase 1
Description of TargetFunctions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha (By similarity). Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.
Uniprot IDQ14914
Protein Accession #NP_001139580.1
Nucleotide Accession #NM_001146108.1
Protein Size (# AA)329
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