Catalog No: OKCA00964
Size:96 Wells
Price: $645.00
SKU
OKCA00964
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology PPIA ELISA Kit (Rat) (OKCA00964) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for PPIA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPIA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PPIA captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 125 pg/mL-8,000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level PPIA were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level PPIA were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Lysates, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 31.25 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | PPIA |
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Gene Full Name | Peptidyl-prolyl cis-trans isomerase A |
Alias Symbols | Cyclophilin 18, Cyclophilin A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Rotamase A |
NCBI Gene Id | 100008724 |
Protein Name | Peptidyl-prolyl cis-trans isomerase A |
Description of Target | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
Uniprot ID | Q9TTC6 |
Protein Accession # | NP_001075526.1 |
Nucleotide Accession # | NM_001082057.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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