Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCA00964
Size:96 Wells
Price: $645.00
SKU
OKCA00964
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat103101-105
1:29693-98
1:48984-94
1:89896-100
ELISA Kit PrincipleAviva Systems Biology PPIA ELISA Kit (Rat) (OKCA00964) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for PPIA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPIA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PPIA captured in well.
ELISA Kit Range125 pg/mL-8,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)106103-109
EDTA Plasma (n=4)9286-98
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level PPIA were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level PPIA were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
PPIA Microplate96 Wells (12 x 8 Well strips)
PPIA Lyophilized Standard2
100X Biotinylated PPIA Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Lysates, Tissue Homogenates
Sensitivity31.25 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPPIA
Gene Full NamePeptidyl-prolyl cis-trans isomerase A
Alias SymbolsCyclophilin 18, Cyclophilin A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Rotamase A
NCBI Gene Id100008724
Protein NamePeptidyl-prolyl cis-trans isomerase A
Description of TargetPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Uniprot IDQ9TTC6
Protein Accession #NP_001075526.1
Nucleotide Accession #NM_001082057.1
Write Your Own Review
You're reviewing:PPIA ELISA Kit (Rat) (OKCA00964)
Your Rating
We found other products you might like!