PPIA ELISA Kit (Human) (OKCD01556)

Catalog No: OKCD01556 (Formerly GWB-KBBPD2)

Size:96WELLS

Human PPIA ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	83-98%	95-104%	90-104%	86-102%
  EDTA Plasma (n=5)	96-103%	87-95%	91-98%	86-98%
  Heparin Plasma (n=5)	95-104%	88-102%	81-102%	91-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin A (CYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin A (CYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin A (CYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclophilin A (CYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.25-80ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	85-99	89
  EDTA Plasma (n=5)	96-105	101
  Heparin Plasma (n=5)	80-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclophilin A (CYPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclophilin A (CYPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PPIA Microplate: 96 Wells (12 x 8 Well Strips)
  • PPIA Lyophilized Standard: 2 x 80 ng
  • 100X Biotinylated PPIA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Sensitivity: < 0.49 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA). No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PPIA
• Gene Full Name: peptidylprolyl isomerase A
• Alias Symbols: Cyclophilin A;cyclosporin A-binding protein;CYPA;CYPH;epididymis secretory sperm binding protein Li 69p;HEL-S-69p;peptidyl-prolyl cis-trans isomerase A;peptidylprolyl isomerase A (cyclophilin A);PPIase A;rotamase A;T cell cyclophilin.
• NCBI Gene Id: 5478
• Protein Name: Peptidyl-prolyl cis-trans isomerase A
• Description of Target: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
• Uniprot ID: P62937
• Protein Accession #: NP_001287910.1
• Nucleotide Accession #: NM_001300981.1
• Protein Size (# AA): 165