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Catalog No: OKCD01817 (Formerly GWB-KBBPY2)
Size:96WELLS
Price: $675.00
SKU
OKCD01817
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Datasheets/ManualsPrintable datasheet for PLOD3 ELISA Kit (Human) (OKCD01817)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PLOD3 Microplate96 Wells (12 x 8 Well Strips)
PLOD3 Lyophilized Standard2 x 20 ng
100X Biotinylated PLOD3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.106 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3). No significant cross-reactivity or interference between Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPLOD3
Gene Full Nameprocollagen-lysine,2-oxoglutarate 5-dioxygenase 3
Alias SymbolsLH3;lysine hydroxylase 3;lysyl hydroxlase 3;lysyl hydroxylase 3;multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3.
NCBI Gene Id8985
Protein NameProcollagen-lysine,2-oxoglutarate 5-dioxygenase 3
Description of TargetForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Uniprot IDO60568
Protein Accession #NP_001075.1
Nucleotide Accession #NM_001084.4
Protein Size (# AA)738
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