Catalog No: OKCD01551 (Formerly GWB-KBBOM6)
Size:96WELLS
Price: $650.00
SKU
OKCD01551
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for PDP1 ELISA Kit (Human) (OKCD01551) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Phosphatase (PDP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Phosphatase (PDP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Phosphatase (PDP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pyruvate Dehydrogenase Phosphatase (PDP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Phosphatase (PDP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Phosphatase (PDP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.125 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Phosphatase (PDP). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Phosphatase (PDP) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | PDP1 |
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Gene Full Name | pyruvate dehydrogenase phosphatase catalytic subunit 1 |
Alias Symbols | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial;PDH;PDP;PDP 1;PDPC;PDPC 1;PPM2A;PPM2C;Protein phosphatase 2C;protein phosphatase 2C, magnesium-dependent, catalytic subunit;protein phosphatase, Mg2+/Mn2+ dependent 2A;pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase;Pyruvate dehydrogenase phosphatase catalytic subunit 1;pyruvate dehyrogenase phosphatase catalytic subunit 1. |
NCBI Gene Id | 54704 |
Protein Name | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial |
Description of Target | Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. |
Uniprot ID | Q9P0J1 |
Protein Accession # | NP_001155251.1 |
Nucleotide Accession # | NM_001161779.1 |
Protein Size (# AA) | 537 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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