Oprk1 ELISA Kit (Mouse) (OKCD01735)

Catalog No: OKCD01735 (Formerly GWB-KBBZV1)

Size:96 Wells

Mouse Oprk1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-92%	87-99%	84-96%	82-105%

• ELISA Kit Principle: Aviva Systems Biology Oprk1 ELISA Kit (Mouse) (OKCD01735) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Oprk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Oprk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Oprk1 captured in the well.
• ELISA Kit Range: 0.781 - 50 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	87-94	91

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Oprk1 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Oprk1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-Oprk1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Oprk1 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated Oprk1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.28 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Opioid Receptor Kappa 1 (OPRk1). No significant cross-reactivity or interference between Opioid Receptor Kappa 1 (OPRk1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: OPRK1
• Gene Full Name: Opioid receptor, kappa 1
• Alias Symbols: Op, R2, KOR, R21, KOR-1, MSL-1, Oprk2, K-OR-1
• NCBI Gene Id: 18387
• Protein Name: Kappa-type opioid receptor
• Description of Target: G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
• Uniprot ID: P33534
• Protein Accession #: NP_001191300.1
• Nucleotide Accession #: NM_001204371.1
• Protein Size (# AA): 380