Catalog No: OKCD01878 (Formerly GWB-KBBVG9)
Size:96WELLS
Price: $675.00
SKU
OKCD01878
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Email: info@avivasysbio.com
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OSBPL8 ELISA Kit (Human) (OKCD01878) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxysterol Binding Protein Like Protein 8 (OSBPL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxysterol Binding Protein Like Protein 8 (OSBPL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxysterol Binding Protein Like Protein 8 (OSBPL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oxysterol Binding Protein Like Protein 8 (OSBPL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxysterol Binding Protein Like Protein 8 (OSBPL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxysterol Binding Protein Like Protein 8 (OSBPL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.138 ng/mL. | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Oxysterol Binding Protein Like Protein 8 (OSBPL8). No significant cross-reactivity or interference between Oxysterol Binding Protein Like Protein 8 (OSBPL8) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | OSBPL8 |
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Gene Full Name | oxysterol binding protein like 8 |
Alias Symbols | MST120;MSTP120;ORP8;OSBP10;OSBP-related protein 8;oxysterol-binding protein-related protein 8. |
NCBI Gene Id | 114882 |
Protein Name | Oxysterol-binding protein-related protein 8 |
Description of Target | Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:26206935). Binds oxysterol, 25-hydroxycholesterol and cholesterol (PubMed:17428193, PubMed:17991739, PubMed:21698267). |
Uniprot ID | Q9BZF1 |
Protein Accession # | NP_001003712.1 |
Nucleotide Accession # | NM_001003712.1 |
Protein Size (# AA) | 889 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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