Nrf1 ELISA Kit (Mouse) (OKCD01688)

Catalog No: OKCD01688 (Formerly GWB-KBBOC6)

Size:96WELLS

Mouse Nrf1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	97-105%	79-90%	86-93%	83-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Respiratory Factor 1 (NRF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Respiratory Factor 1 (NRF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Respiratory Factor 1 (NRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Respiratory Factor 1 (NRF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	95-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Respiratory Factor 1 (NRF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Respiratory Factor 1 (NRF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Nrf1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Nrf1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Nrf1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Respiratory Factor 1 (NRF1). No significant cross-reactivity or interference between Nuclear Respiratory Factor 1 (NRF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Nrf1
• Gene Full Name: nuclear respiratory factor 1
• Alias Symbols: alpha palindromic-binding protein;alpha-pal;C87038;D6Ertd415;D6Ertd415e;NRF-1;nuclear respiratory factor 1.
• NCBI Gene Id: 18181
• Protein Name: Nuclear respiratory factor 1
• Description of Target: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).
• Uniprot ID: Q9WU00
• Protein Accession #: NP_001157698.1
• Nucleotide Accession #: NM_001164226.1
• Protein Size (# AA): 503