NPC1 ELISA Kit (Mouse) (OKCA00909)

Catalog No: OKCA00909

Size:96 Wells

Mouse NPC1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	96	90-100
  1:2	93	89-97
  1:4	99	94-103
  1:8	89	85-93

• ELISA Kit Principle: Aviva Systems Biology Npc1 ELISA Kit (Mouse) (OKCA00909) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Npc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Npc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Npc1 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	105	100-112
  EDTA Plasma (n=4)	90	85-94

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Npc1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Npc1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Npc1 Microplate: 96 Wells (12 x 8 Well strips)
  • Npc1 Lyophilized Standard: 2
  • 100X Biotinylated Npc1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPC1
• Gene Full Name: Niemann-Pick type C1
• Alias Symbols: lc, spm, lcsd, nmf16, C85354, nmf164, D18Ertd139, D18Ertd723, D18Ertd139e, D18Ertd723e, A430089E03Rik
• NCBI Gene Id: 18145
• Protein Name: Niemann-Pick C1 protein
• Description of Target: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.
• Uniprot ID: O35604
• Protein Accession #: NP_032746.2
• Nucleotide Accession #: NM_008720.2