Catalog No: OKCD01704 (Formerly GWB-KBBXY2)
Size:96WELLS
Price: $675.00
SKU
OKCD01704
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Datasheets/ManualsPrintable datasheet for NTSR1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotensin Receptor 1, High Affinity (NTSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neurotensin Receptor 1, High Affinity (NTSR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NTSR1 Microplate96 Wells (12 x 8 Well Strips)
NTSR1 Lyophilized Standard2 x 10 ng
100X Biotinylated NTSR1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurotensin Receptor 1, High Affinity (NTSR1). No significant cross-reactivity or interference between Neurotensin Receptor 1, High Affinity (NTSR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNTSR1
Gene Full NameNeurotensin receptor 1
Alias SymbolsNTR
NCBI Gene Id4923
Protein NameNeurotensin receptor type 1
Description of TargetG-protein coupled receptor for the tridecapeptide neurotensin (NTS).
Uniprot IDP30989
Protein Accession #NP_002522.2
Nucleotide Accession #NM_002531.2
Protein Size (# AA)418
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