Catalog No: OKCA00987
Size:96 Wells
Price: $645.00
SKU
OKCA00987
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9592-104
1:29792-103
1:49389-95
1:88682-92
ELISA Kit PrincipleAviva Systems Biology NPY ELISA Kit (Rat) (OKCA00987) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for NPY has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPY captured in well.
ELISA Kit Range1.56 pg/mL-100 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9387-99
EDTA Plasma (n=4)105100-110
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level NPY were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level NPY were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
NPY Microplate96 Wells (12 x 8 Well strips)
NPY Lyophilized Standard2
100X Biotinylated NPY Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.39 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNPY
Gene Full NameNeuropeptide Y
Protein NameNeuropeptide Y
Description of TargetNPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Uniprot IDP68006
Protein Accession #NP_001153758.1
Nucleotide Accession #NM_001160286.1
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