N4BP2 ELISA Kit (Human) (OKCD02113)

Catalog No: OKCD02113 (Formerly GWB-KBBDQ9)

Size:96WELLS

Human N4BP2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	82-97%	83-97%	79-93%	80-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEDD4 Binding Protein 2 (N4BP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEDD4 Binding Protein 2 (N4BP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEDD4 Binding Protein 2 (N4BP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEDD4 Binding Protein 2 (N4BP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	80-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEDD4 Binding Protein 2 (N4BP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEDD4 Binding Protein 2 (N4BP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-N4BP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • N4BP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated N4BP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEDD4 Binding Protein 2 (N4BP2). No significant cross-reactivity or interference between NEDD4 Binding Protein 2 (N4BP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: N4BP2
• Gene Full Name: NEDD4 binding protein 2
• Alias Symbols: B3BP;BCL-3-binding protein;NEDD4-binding protein 2.
• NCBI Gene Id: 55728
• Protein Name: NEDD4-binding protein 2
• Description of Target: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair or recombination.
• Uniprot ID: Q86UW6
• Protein Accession #: NP_001305288.1
• Nucleotide Accession #: NM_001318359.1
• Protein Size (# AA): 1770