Catalog No: OKCD01538 (Formerly GWB-KBBRJ3)
Size:96WELLS
Price: $625.00
SKU
OKCD01538
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Datasheets/ManualsPrintable datasheet for Mt3 ELISA Kit (Mouse) (OKCD01538)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-94%78-94%90-104%95-104%
EDTA Plasma (n=5)91-103%87-101%86-93%79-92%
Heparin Plasma (n=5)97-105%86-98%99-105%82-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metallothionein 3 (MT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metallothionein 3 (MT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metallothionein 3 (MT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Metallothionein 3 (MT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-10398
EDTA Plasma (n=5)90-9894
Heparin Plasma (n=5)96-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metallothionein 3 (MT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metallothionein 3 (MT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Mt3 Microplate96 Wells (12 x 8 Well Strips)
Mt3 Lyophilized Standard2 x 10 ng
100X Biotinylated Mt3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.074 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Metallothionein 3 (MT3). No significant cross-reactivity or interference between Metallothionein 3 (MT3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMt3
Gene Full Namemetallothionein 3
Alias SymbolsGIF;growth inhibitory factor;metallothionein-3;metallothionein-III;Mt-3;MT-III.
NCBI Gene Id17751
Protein NameMetallothionein-3
Description of TargetBinds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro (By similarity).
Uniprot IDP28184
Protein Accession #NP_038631.1
Nucleotide Accession #NM_013603.2
Protein Size (# AA)68
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