Catalog No: OKCD01560 (Formerly GWB-KBBWO5)
Size:96WELLS
Price: $650.00
SKU
OKCD01560
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Datasheets/ManualsPrintable datasheet for Mpz ELISA Kit (Rat) (OKCD01560)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Protein Zero, Myelin (MPZ) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Protein Zero, Myelin (MPZ) and unlabeled Protein Zero, Myelin (MPZ) (Standards or samples) with the pre-coated antibody specific to Protein Zero, Myelin (MPZ). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Protein Zero, Myelin (MPZ) in the sample.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Mpz Microplate96 Wells (12 x 8 Well Strips)
Mpz Lyophilized Standard2 x 20 ng
100X Biotinylated Mpz Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.122 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Zero, Myelin (MPZ). No significant cross-reactivity or interference between Protein Zero, Myelin (MPZ) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMpz
Gene Full Namemyelin protein zero
Alias SymbolsMPP;myelin peripheral protein;myelin protein P0;Myelin protein zero;myelin protein zero (Charcot-Marie-Tooth neuropathy 1B);P0.
NCBI Gene Id24564
Protein NameMyelin protein P0
Description of TargetIs an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
Uniprot IDP06907
Protein Accession #NP_058723.2
Nucleotide Accession #NM_017027.2
Protein Size (# AA)248
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