Mmp1a ELISA Kit (Mouse) (OKCD01468)

Catalog No: OKCD01468 (Formerly GWB-KBBEW4)

Size:96WELLS

Mouse Mmp1a ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-97%	81-94%	81-104%	85-99%
  Heparin Plasma (n=5)	86-97%	90-104%	98-105%	89-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1 (MMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1 (MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 1 (MMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 1 (MMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	93-102	97
  Heparin Plasma (n=5)	83-91	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mmp1a Microplate: 96 Wells (12 x 8 Well Strips)
  • Mmp1a Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated Mmp1a Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 27.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 1 (MMP1). No significant cross-reactivity or interference between Matrix Metalloproteinase 1 (MMP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Mmp1a
• Gene Full Name: matrix metallopeptidase 1a (interstitial collagenase)
• Alias Symbols: collagenase-like A;interstitial collagenase A;matrix metalloproteinase 1a (interstitial collagenase);Matrix metalloproteinase-1a;Mco;Mcola;Mcol-A;MMP-1a.
• NCBI Gene Id: 83995
• Protein Name: Interstitial collagenase A
• Description of Target: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (By similarity). Able to degrade synthetic peptides and type I and II fibrillar collagen.
• Uniprot ID: Q9EPL5
• Protein Accession #: NP_114395.1
• Nucleotide Accession #: NM_032006.3
• Protein Size (# AA): 464