MYH6 ELISA Kit (Human) (OKCD01760)

Catalog No: OKCD01760 (Formerly GWB-KBBVR6)

Size:96WELLS

Human MYH6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	82-91%	79-102%	94-105%	97-105%
  EDTA Plasma (n=5)	92-99%	97-104%	78-89%	80-93%
  Heparin Plasma (n=5)	98-105%	92-101%	87-97%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	82-103	86
  EDTA Plasma (n=5)	95-102	99
  Heparin Plasma (n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MYH6 Microplate: 96 Wells (12 x 8 Well Strips)
  • MYH6 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MYH6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). No significant cross-reactivity or interference between Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MYH6
• Gene Full Name: myosin heavy chain 6
• Alias Symbols: alpha-MHC;ASD3;CMD1EE;CMH14;MYHC;MYHCA;myHC-alpha;Myosin heavy chain 6;Myosin heavy chain, cardiac muscle alpha isoform;myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1);myosin-6;SSS3.
• NCBI Gene Id: 4624
• Protein Name: Myosin-6
• Description of Target: Muscle contraction.
• Uniprot ID: P13533
• Protein Accession #: NP_002462.2
• Nucleotide Accession #: NM_002471.3
• Protein Size (# AA): 1939