MXRA5 ELISA Kit (Human) (OKCD02088)

Catalog No: OKCD02088 (Formerly GWB-KBBXT7)

Size:96WELLS

Human MXRA5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-102%	90-98%	89-98%	85-105%
  EDTA Plasma (n=5)	87-94%	91-101%	97-105%	81-99%
  Heparin Plasma (n=5)	78-91%	85-94%	78-94%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Remodelling Associated Protein 5 (MXRA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Remodelling Associated Protein 5 (MXRA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Remodelling Associated Protein 5 (MXRA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Remodelling Associated Protein 5 (MXRA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	85-93	89
  EDTA Plasma (n=5)	94-101	98
  Heparin Plasma (n=5)	86-97	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Remodelling Associated Protein 5 (MXRA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Remodelling Associated Protein 5 (MXRA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MXRA5 Microplate: 96 Wells (12 x 8 Well Strips)
  • MXRA5 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated MXRA5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 26.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Remodelling Associated Protein 5 (MXRA5). No significant cross-reactivity or interference between Matrix Remodelling Associated Protein 5 (MXRA5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MXRA5
• Gene Full Name: matrix remodeling associated 5
• Alias Symbols: adhesion protein with leucine-rich repeats and Immunoglobulin domains related to perlecan;adlican;matrix-remodeling-associated protein 5;matrix-remodelling associated 5.
• NCBI Gene Id: 25878
• Protein Name: Matrix-remodeling-associated protein 5
• Description of Target: In kidney, has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli.
• Uniprot ID: Q9NR99
• Protein Accession #: NP_056234.2
• Nucleotide Accession #: NM_015419.3
• Protein Size (# AA): 2828