MUC6 ELISA Kit (Human) (OKCD01588)

Catalog No: OKCD01588 (Formerly GWB-KBBKK9)

Size:96WELLS

Human MUC6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-89%	96-105%	80-95%	80-101%
  EDTA Plasma (n=5)	84-91%	94-102%	78-97%	80-96%
  Heparin Plasma (n=5)	84-94%	81-88%	85-102%	88-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 6 (MUC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 6 (MUC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 6 (MUC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mucin 6 (MUC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-101	92
  EDTA Plasma (n=5)	84-98	94
  Heparin Plasma (n=5)	87-94	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 6 (MUC6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 6 (MUC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MUC6 Microplate: 96 Wells (12 x 8 Well Strips)
  • MUC6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated MUC6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 6 (MUC6). No significant cross-reactivity or interference between Mucin 6 (MUC6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MUC6
• Gene Full Name: mucin 6, oligomeric mucus/gel-forming
• Alias Symbols: gastric mucin-6;MUC-6;mucin-6.
• NCBI Gene Id: 4588
• Protein Name: Mucin-6
• Description of Target: May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
• Uniprot ID: Q6W4X9
• Protein Accession #: NP_005952.2
• Nucleotide Accession #: NM_005961.2
• Protein Size (# AA): 2439