MS4A1 ELISA Kit (Human) (OKCD01568)

Catalog No: OKCD01568 (Formerly GWB-KBBHU1)

Size:96WELLS

Human MS4A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-105%	97-104%	95-105%	89-101%
  EDTA Plasma (n=5)	95-104%	90-104%	80-101%	90-104%
  Heparin Plasma (n=5)	85-104%	93-105%	87-96%	78-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.25-80ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-102	85
  EDTA Plasma (n=5)	90-98	95
  Heparin Plasma (n=5)	78-104	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Spanning 4 Domains Subfamily A, Member 1 (CD20) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MS4A1 Microplate: 96 Wells (12 x 8 Well Strips)
  • MS4A1 Lyophilized Standard: 2 x 80 ng
  • 100X Biotinylated MS4A1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.49 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1). No significant cross-reactivity or interference between Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MS4A1
• Gene Full Name: membrane spanning 4-domains A1
• Alias Symbols: B1;B-lymphocyte antigen CD20;B-lymphocyte cell-surface antigen B1;B-lymphocyte surface antigen B1;Bp35;CD20;CD20 antigen;CD20 receptor;CVID5;FMC7;LEU-16;leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;membrane-spanning 4-domains, subfamily A, member 1;S7.
• NCBI Gene Id: 931
• Protein Name: B-lymphocyte antigen CD20
• Description of Target: This protein may be involved in the regulation of B-cell activation and proliferation.
• Uniprot ID: P11836
• Protein Accession #: NP_068769.2
• Nucleotide Accession #: NM_021950.3
• Protein Size (# AA): 297