MGAT5 ELISA Kit (Human) (OKCD01965)

Catalog No: OKCD01965 (Formerly GWB-KBBZF4)

Size:96 Wells

Human MGAT5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	94-102%	99-105%	82-93%	90-97%
  EDTA Plasma (n=5)	96-103%	96-104%	82-96%	96-104%
  Heparin Plasma (n=5)	84-101%	90-103%	98-105%	92-99%

• ELISA Kit Principle: Aviva Systems Biology MGAT5 ELISA Kit (Human) (OKCD01965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MGAT5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MGAT5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MGAT5 captured in the well.
• ELISA Kit Range: 0.156 - 10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	90-99	95
  EDTA Plasma (n=5)	97-105	101
  Heparin Plasma (n=5)	88-105	95

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level MGAT5 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level MGAT5 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-MGAT5 Microplate: 96 Wells (12 x 8 Well Strips)
  • MGAT5 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MGAT5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5). No significant cross-reactivity or interference between Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MGAT5
• Gene Full Name: Mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
• Alias Symbols: GNT-V, GNT-VA, MGAT5A, glcNAc-T V
• NCBI Gene Id: 4249
• Protein Name: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• Description of Target: Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
• Uniprot ID: Q09328
• Protein Accession #: NP_002401.1
• Nucleotide Accession #: NM_002410.4
• Protein Size (# AA): 741