MANSC1 ELISA Kit (Human) (OKCD02117)

Catalog No: OKCD02117 (Formerly GWB-KBBWH4)

Size:96WELLS

Human MANSC1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-99%	92-101%	98-105%	80-105%
  EDTA Plasma (n=5)	82-96%	99-105%	79-97%	93-101%
  Heparin Plasma (n=5)	86-98%	88-101%	79-95%	86-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MANSC Domain Containing Protein 1 (MANSC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MANSC Domain Containing Protein 1 (MANSC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MANSC Domain Containing Protein 1 (MANSC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MANSC Domain Containing Protein 1 (MANSC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	95-105	99
  EDTA Plasma (n=5)	86-98	91
  Heparin Plasma (n=5)	79-95	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MANSC Domain Containing Protein 1 (MANSC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MANSC Domain Containing Protein 1 (MANSC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MANSC1 Microplate: 96 Wells (12 x 8 Well Strips)
  • MANSC1 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated MANSC1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MANSC Domain Containing Protein 1 (MANSC1). No significant cross-reactivity or interference between MANSC Domain Containing Protein 1 (MANSC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MANSC1
• Gene Full Name: MANSC domain containing 1
• Alias Symbols: 9130403P13Rik;LOH12CR3;loss of heterozygosity 12 chromosomal region 3 protein;MANSC domain-containing protein 1.
• NCBI Gene Id: 54682
• Protein Name: MANSC domain-containing protein 1
• Uniprot ID: Q9H8J5
• Protein Accession #: NP_060520.2
• Nucleotide Accession #: NM_018050.2
• Protein Size (# AA): 431