LPCAT4 ELISA Kit (Human) (OKCD01921)

Catalog No: OKCD01921 (Formerly GWB-KBBUU6)

Size:96WELLS

Human LPCAT4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-96%	80-96%	96-103%	90-104%
  EDTA Plasma (n=5)	85-93%	78-96%	93-104%	86-93%
  Heparin Plasma (n=5)	90-104%	93-103%	90-104%	91-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 4 (LPCAT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	90-103	96
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	90-104	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LPCAT4 Microplate: 96 Wells (12 x 8 Well Strips)
  • LPCAT4 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated LPCAT4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.34 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 4 (LPCAT4). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LPCAT4
• Gene Full Name: Lysophosphatidylcholine acyltransferase 4
• Alias Symbols: AYTL3, AGPAT7, LPEAT2, LPAAT-eta
• NCBI Gene Id: 254531
• Protein Name: Lysophospholipid acyltransferase LPCAT4
• Description of Target: Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidycholine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors.
• Uniprot ID: Q643R3
• Protein Accession #: NP_705841.2
• Nucleotide Accession #: NM_153613.2
• Protein Size (# AA): 524