IRF7 ELISA Kit (Human) (OKCA00703)

Catalog No: OKCA00703

Size:96 Wells

Human IRF7 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	91	86-94
  1:2	101	96-104
  1:4	91	84-95
  1:8	97	92-103

• ELISA Kit Principle: Aviva Systems Biology IRF7 ELISA Kit (Human) (OKCA00703) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IRF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IRF7 captured in the well.
• ELISA Kit Range: 23.4 - 1,500 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93	87-97
  EDTA Plasma (n=4)	94	88-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IRF7 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IRF7 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: < 8%
  Inter-Assay CV: < 10%
• ELISA Kit Component:
  • IRF7 Microplate: 96 Wells (12 x 8 Well strips)
  • IRF7 Lyophilized Standard: 2
  • 100X Biotinylated IRF7 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: < 5.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IRF7
• Gene Full Name: Interferon regulatory factor 7
• Alias Symbols: IMD39, IRF-7, IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H
• NCBI Gene Id: 3665
• Protein Name: Interferon regulatory factor 7
• Description of Target: IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
• Uniprot ID: Q92985
• Protein Accession #: NP_001563.2
• Nucleotide Accession #: NM_001572.3