IMPDH2 ELISA Kit (Human) (OKCD01876)

Catalog No: OKCD01876 (Formerly GWB-KBBVM6)

Size:96WELLS

Human IMPDH2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IMPDH2 Microplate: 96 Wells (12 x 8 Well Strips)
  • IMPDH2 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated IMPDH2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2). No significant cross-reactivity or interference between Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IMPDH2
• Gene Full Name: inosine monophosphate dehydrogenase 2
• Alias Symbols: epididymis secretory sperm binding protein;IMP (inosine 5'-monophosphate) dehydrogenase 2;IMP (inosine monophosphate) dehydrogenase 2;IMP dehydrogenase II;IMP oxireductase 2;IMPD 2;IMPD2;IMPDH 2;IMPDH-II;inosine 5' phosphate dehydrogenase 2;inosine monophosphate dehydrogenase type II;inosine-5'-monophosphate dehydrogenase 2;inosine-5'-monophosphate dehydrogenase type II.
• NCBI Gene Id: 3615
• Protein Name: Inosine-5'-monophosphate dehydrogenase 2
• Description of Target: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
• Uniprot ID: P12268
• Protein Accession #: NP_000875.2
• Nucleotide Accession #: NM_000884.2
• Protein Size (# AA): 514