IL1F10 ELISA Kit (Human) (OKCD02101)

Catalog No: OKCD02101 (Formerly GWB-KBBCD4)

Size:96WELLS

Human IL1F10 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	99-105%	90-99%	93-103%	82-90%
  EDTA Plasma (n=5)	88-98%	92-104%	82-89%	93-101%
  Heparin Plasma (n=5)	98-105%	88-102%	80-89%	85-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Theta (IL1q). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Theta (IL1q). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Theta (IL1q), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Theta (IL1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-97	85
  EDTA Plasma (n=5)	91-101	95
  Heparin Plasma (n=5)	91-103	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Theta (IL1q) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Theta (IL1q) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL1F10 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL1F10 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated IL1F10 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Theta (IL1q). No significant cross-reactivity or interference between Interleukin 1 Theta (IL1q) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL1F10
• Gene Full Name: interleukin 1 family member 10
• Alias Symbols: family of interleukin 1-theta;FIL1 theta;FIL1-theta;FKSG75;IL-1 theta;IL-1F10 (canonical form IL-1F10a);IL1HY2;IL-1HY2;IL1-theta;IL-38;interleukin 1 family member 10 (theta);interleukin-1 family member 10;interleukin-1 HY2;interleukin-1 receptor antagonist FKSG75;interleukin-1 receptor antagonist-like FIL1 theta;Interleukin-1 theta;interleukin-38.
• NCBI Gene Id: 84639
• Protein Name: Interleukin-1 family member 10
• Description of Target: Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2.
• Uniprot ID: Q8WWZ1
• Protein Accession #: NP_115945.4
• Nucleotide Accession #: NM_032556.5
• Protein Size (# AA): 152