IGLL5 ELISA Kit (Human) (OKCD02120)

Catalog No: OKCD02120 (Formerly GWB-KBBBQ8)

Size:96WELLS

Human IGLL5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin Lambda Like Polypeptide 5 (IglL5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin Lambda Like Polypeptide 5 (IglL5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin Lambda Like Polypeptide 5 (IglL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Immunoglobulin Lambda Like Polypeptide 5 (IglL5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin Lambda Like Polypeptide 5 (IglL5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin Lambda Like Polypeptide 5 (IglL5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IGLL5 Microplate: 96 Wells (12 x 8 Well Strips)
  • IGLL5 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated IGLL5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Immunoglobulin Lambda Like Polypeptide 5 (IglL5). No significant cross-reactivity or interference between Immunoglobulin Lambda Like Polypeptide 5 (IglL5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IGLL5
• Gene Full Name: immunoglobulin lambda like polypeptide 5
• Alias Symbols: anti HBs antibody light-chain Fab fragment;G lambda-1;germline immunoglobulin lambda 1;IGL;IGLV;immunoglobin anti-granzymeB light chain variable region;immunoglobulin lambda 1 light chain;immunoglobulin lambda 2 light chain;immunoglobulin lambda 3 light chain;immunoglobulin lambda light chain variable region;immunoglobulin lambda-3 surrogate light chain;immunoglobulin lambda-like polypeptide 5;immunoglobulin light chain variable region EM1-PPS-4-L1-1;omega light chain protein 14.1;VL-MAR.
• NCBI Gene Id: 100423062
• Protein Name: Immunoglobulin lambda-like polypeptide 5
• Description of Target: This gene encodes one of the immunoglobulin lambda-like polypeptides. It is located within the immunoglobulin lambda locus but it does not require somatic rearrangement for expression. The first exon of this gene is unrelated to immunoglobulin variable genes; the second and third exons are the immunoglobulin lambda joining 1 and the immunoglobulin lambda constant 1 gene segments. Alternative splicing results in multiple transcript variants.
• Uniprot ID: B9A064
• Protein Accession #: NP_001171597.1
• Nucleotide Accession #: NM_001178126.1
• Protein Size (# AA): 214