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Catalog No: OKCD02051 (Formerly GWB-KBBZB8)
Size:96WELLS
Price: $750.00
SKU
OKCD02051
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Datasheets/ManualsPrintable datasheet for IGF2BP3 ELISA Kit (Human) (OKCD02051)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-97%95-103%79-98%83-98%
EDTA Plasma (n=5)86-102%82-91%85-97%88-102%
Heparin Plasma (n=5)79-96%88-95%95-103%78-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)81-9892
EDTA Plasma (n=5)88-10292
Heparin Plasma (n=5)88-9792
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IGF2BP3 Microplate96 Wells (12 x 8 Well Strips)
IGF2BP3 Lyophilized Standard2 x 10 ng
100X Biotinylated IGF2BP3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIGF2BP3
Gene Full Nameinsulin like growth factor 2 mRNA binding protein 3
Alias Symbolscancer/testis antigen 98;CT98;IGF2 mRNA-binding protein 3;IGF-II mRNA-binding protein 3;IMP3;IMP-3;insulin-like growth factor 2 mRNA-binding protein 3;KH domain containing protein overexpressed in cancer;KH domain-containing protein overexpressed in cancer;KOC;KOC1;VICKZ family member 3;VICKZ3.
NCBI Gene Id10643
Protein NameInsulin-like growth factor 2 mRNA-binding protein 3
Description of TargetRNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Uniprot IDO00425
Protein Accession #NP_006538.2
Nucleotide Accession #NM_006547.2
Protein Size (# AA)579
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