HRSP12 ELISA Kit (Human) (OKCD01975)

Catalog No: OKCD01975 (Formerly GWB-KBBHH6)

Size:96WELLS

Human HRSP12 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	94-102%	83-90%	94-105%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Responsive Protein 12 (HRSP12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Responsive Protein 12 (HRSP12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Responsive Protein 12 (HRSP12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Responsive Protein 12 (HRSP12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	91-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Responsive Protein 12 (HRSP12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Responsive Protein 12 (HRSP12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HRSP12 Microplate: 96 Wells (12 x 8 Well Strips)
  • HRSP12 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HRSP12 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Responsive Protein 12 (HRSP12). No significant cross-reactivity or interference between Heat Responsive Protein 12 (HRSP12) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HRSP12
• Gene Full Name: Reactive intermediate imine deaminase A homolog
• Alias Symbols: PSP, P14.5, UK114, HRSP12, hp14.5
• NCBI Gene Id: 10247
• Protein Name: Ribonuclease UK114
• Description of Target: Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA.
• Uniprot ID: P52758
• Protein Accession #: NP_005827.1
• Nucleotide Accession #: NM_005836.2
• Protein Size (# AA): 137