HIST1H1A ELISA Kit (Human) (OKCD02016)

Catalog No: OKCD02016 (Formerly GWB-KBBES3)

Size:96WELLS

Human HIST1H1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	83-103%	97-105%	82-104%	78-88%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H1a (HIST1H1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 1, H1a (HIST1H1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 1, H1a (HIST1H1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 1, H1a (HIST1H1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	87-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 1, H1a (HIST1H1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 1, H1a (HIST1H1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HIST1H1A Microplate: 96 Wells (12 x 8 Well Strips)
  • HIST1H1A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated HIST1H1A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Cluster 1, H1a (HIST1H1A). No significant cross-reactivity or interference between Histone Cluster 1, H1a (HIST1H1A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HIST1H1A
• Gene Full Name: Histone cluster 1 H1 family member a
• Alias Symbols: H1A, H1.1, H1F1, HIST1, HIST1H1A
• NCBI Gene Id: 3024
• Protein Name: Histone H1.1
• Description of Target: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
• Uniprot ID: Q02539
• Protein Accession #: NP_005316.1
• Nucleotide Accession #: NM_005325.3
• Protein Size (# AA): 215