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Catalog No: OKCD01631 (Formerly GWB-KBBRA8)
Size:96WELLS
Price: $725.00
SKU
OKCD01631
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Datasheets/ManualsPrintable datasheet for Gdf3 ELISA Kit (Rat) (OKCD01631)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-89%94-101%94-105%92-101%
EDTA Plasma (n=5)79-105%80-94%80-97%81-99%
Heparin Plasma (n=5)82-99%97-104%80-101%94-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 3 (GDF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 3 (GDF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 3 (GDF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Differentiation Factor 3 (GDF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range62.50-4000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-10197
EDTA Plasma (n=5)91-105101
Heparin Plasma (n=5)78-9389
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 3 (GDF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 3 (GDF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Gdf3 Microplate96 Wells (12 x 8 Well Strips)
Gdf3 Lyophilized Standard2 x 4 ng
100X Biotinylated Gdf3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 29.7 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 3 (GDF3). No significant cross-reactivity or interference between Growth Differentiation Factor 3 (GDF3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGdf3
Gene Full Namegrowth differentiation factor 3
Alias Symbolsgrowth/differentiation factor 3;RGD1564178.
NCBI Gene Id500311
Protein NameGrowth differentiation factor 3
Description of TargetGrowth differentiation factor 3
Uniprot IDA2SY89
Protein Accession #NP_001103141.1
Nucleotide Accession #NM_001109671.1
Protein Size (# AA)366
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