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Catalog No: OKCD01813 (Formerly GWB-KBBVU8)
Size:96WELLS
Price: $675.00
SKU
OKCD01813
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Datasheets/ManualsPrintable datasheet for GJb6 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-105%92-99%86-94%86-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gap Junction Protein Beta 6 (GJb6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gap Junction Protein Beta 6 (GJb6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gap Junction Protein Beta 6 (GJb6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gap Junction Protein Beta 6 (GJb6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum87-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gap Junction Protein Beta 6 (GJb6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gap Junction Protein Beta 6 (GJb6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GJB6 Microplate96 Wells (12 x 8 Well Strips)
GJB6 Lyophilized Standard2 x 20 ng
100X Biotinylated GJB6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.127 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Gap Junction Protein Beta 6 (GJb6). No significant cross-reactivity or interference between Gap Junction Protein Beta 6 (GJb6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGJB6
Gene Full NameGap junction protein beta 6
Alias SymbolsED2, EDH, HED, CX30, HED2, DFNA3, ECTD2, DFNA3B, DFNB1B
NCBI Gene Id10804
Protein NameGap junction beta-6 protein
Description of TargetOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Uniprot IDO95452
Protein Accession #NP_001103689.1
Nucleotide Accession #NM_001110219.2
Protein Size (# AA)261
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