GARS ELISA Kit (Human) (OKCD01731)

Catalog No: OKCD01731 (Formerly GWB-KBBKY9)

Size:96WELLS

Human GARS ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-98%	99-105%	93-102%	78-94%
  EDTA Plasma (n=5)	80-95%	84-102%	95-102%	90-98%
  Heparin Plasma (n=5)	94-101%	97-104%	96-104%	83-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-98	94
  EDTA Plasma (n=5)	94-103	98
  Heparin Plasma (n=5)	90-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GARS Microplate: 96 Wells (12 x 8 Well Strips)
  • GARS Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated GARS Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycyl tRNA Synthetase (GARS). No significant cross-reactivity or interference between Glycyl tRNA Synthetase (GARS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GARS1
• Gene Full Name: glycyl-tRNA synthetase 1
• Alias Symbols: ap4A synthetase;AP-4-A synthetase;Charcot-Marie-Tooth neuropathy 2D;Charcot-Marie-Tooth neuropathy, neuronal type, D;CMT2D;diadenosine tetraphosphate synthetase;DSMAV;GARS;glycine--tRNA ligase;Glycyl-tRNA synthetase;Glycyl-tRNA synthetase 1;GlyRS;HMN5;HMN5A;SMAD1;SMAJI.
• NCBI Gene Id: 2617
• Protein Name: Glycine--tRNA ligase
• Description of Target: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:28675565, PubMed:24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017).
• Uniprot ID: P41250
• Protein Accession #: NP_001303701.1
• Nucleotide Accession #: NM_001316772.1
• Protein Size (# AA): 739