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Catalog No: OKCD01713 (Formerly GWB-KBBBI8)
Size:96WELLS
Price: $700.00
SKU
OKCD01713
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Datasheets/ManualsPrintable datasheet for Fgf18 ELISA Kit (Mouse) (OKCD01713)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-104%80-91%92-102%80-89%
EDTA Plasma (n=5)96-105%99-105%96-103%79-97%
Heparin Plasma (n=5)79-98%85-92%94-104%80-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 18 (FGF18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 18 (FGF18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 18 (FGF18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 18 (FGF18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)96-104101
EDTA Plasma (n=5)97-105101
Heparin Plasma (n=5)91-9995
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Fgf18 Microplate96 Wells (12 x 8 Well Strips)
Fgf18 Lyophilized Standard2 x 1 ng
100X Biotinylated Fgf18 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18). No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFgf18
Gene Full Namefibroblast growth factor 18
Alias SymbolsD130055P09Rik;FGF-18;Fgf6a;fibroblast growth factor 18;fibroblast growth factor 6a;zFGF5.
NCBI Gene Id14172
Protein NameFibroblast growth factor 18
Description of TargetPlays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation (By similarity).
Uniprot IDO89101
Protein Accession #NP_032031.1
Nucleotide Accession #NM_008005.2.
Protein Size (# AA)207
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