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Catalog No: OKCD01824 (Formerly GWB-KBBNN8)
Size:96WELLS
Price: $675.00
SKU
OKCD01824
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Datasheets/ManualsPrintable datasheet for FZD6 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Homolog 6 (FZD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Homolog 6 (FZD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Homolog 6 (FZD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Frizzled Homolog 6 (FZD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Frizzled Homolog 6 (FZD6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Frizzled Homolog 6 (FZD6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-FZD6 Microplate96 Wells (12 x 8 Well Strips)
FZD6 Lyophilized Standard2 x 20 ng
100X Biotinylated FZD6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.119 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Frizzled Homolog 6 (FZD6). No significant cross-reactivity or interference between Frizzled Homolog 6 (FZD6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolFZD6
Gene Full NameFrizzled class receptor 6
Alias SymbolsFZ6, FZ-6, HFZ6, NDNC1, NDNC10
NCBI Gene Id8323
Protein NameFrizzled-6
Description of TargetReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
Uniprot IDO60353
Protein Accession #NP_001158087.1
Nucleotide Accession #NM_001164615.1
Protein Size (# AA)706
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