EPO ELISA Kit (Horse) (OKCD01439)

Catalog No: OKCD01439 (Formerly GWB-KBBAJ1)

Size:96WELLS

Horse EPO ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Horse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	95-103%	96-104%	92-105%	80-94%
  EDTA Plasma (n=5)	79-101%	78-98%	89-103%	78-102%
  Heparin Plasma (n=5)	97-105%	80-101%	81-96%	96-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythropoietin (EPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Erythropoietin (EPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	86-94	89
  EDTA Plasma (n=5)	80-91	88
  Heparin Plasma (n=5)	88-97	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EPO Microplate: 96 Wells (12 x 8 Well Strips)
  • EPO Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated EPO Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 11.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO). No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EPO
• Alias Symbols: Erythropoietin
• NCBI Gene Id: 100033849
• Protein Name: Erythropoietin
• Description of Target: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
• Uniprot ID: Q867B1
• Protein Accession #: NP_001075294.1
• Nucleotide Accession #: NM_001081825.1
• Protein Size (# AA): 192