Catalog No: OKCD02123 (Formerly GWB-KBBAE3)
Size:96WELLS
Price: $750.00
SKU
OKCD02123
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Datasheets/ManualsPrintable datasheet for ENDOD1 ELISA Kit (Human) (OKCD02123)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-89%87-96%86-94%81-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endonuclease Domain Containing Protein 1 (ENDOD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endonuclease Domain Containing Protein 1 (ENDOD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endonuclease Domain Containing Protein 1 (ENDOD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endonuclease Domain Containing Protein 1 (ENDOD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum80-9084
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endonuclease Domain Containing Protein 1 (ENDOD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endonuclease Domain Containing Protein 1 (ENDOD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ENDOD1 Microplate96 Wells (12 x 8 Well Strips)
ENDOD1 Lyophilized Standard2 x 100 ng
100X Biotinylated ENDOD1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.54 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Endonuclease Domain Containing Protein 1 (ENDOD1). No significant cross-reactivity or interference between Endonuclease Domain Containing Protein 1 (ENDOD1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolENDOD1
Gene Full Nameendonuclease domain containing 1
Alias Symbolsendonuclease domain-containing 1 protein.
NCBI Gene Id23052
Protein NameEndonuclease domain-containing 1 protein
Description of TargetMay act as a DNase and a RNase.
Uniprot IDO94919
Protein Accession #NP_055851.1
Nucleotide Accession #NM_015036.2
Protein Size (# AA)500
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