Catalog No: OKCD01804 (Formerly GWB-KBBVQ3)
Size:96WELLS
Price: $675.00
SKU
OKCD01804
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Datasheets/ManualsPrintable datasheet for EFNB1 ELISA Kit (Human) (OKCD01804)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-99%94-103%86-96%81-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin B1 (EFNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin B1 (EFNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin B1 (EFNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ephrin B1 (EFNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum93-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin B1 (EFNB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin B1 (EFNB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EFNB1 Microplate96 Wells (12 x 8 Well Strips)
EFNB1 Lyophilized Standard2 x 10 ng
100X Biotinylated EFNB1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ephrin B1 (EFNB1). No significant cross-reactivity or interference between Ephrin B1 (EFNB1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEFNB1
Gene Full Nameephrin B1
Alias SymbolsCFND;CFNS;EFB1;EFL3;EFL-3;ELK ligand;Elk-L;eph-related receptor tyrosine kinase ligand 2;ephrin-B1;EPLG2;LERK2.
NCBI Gene Id1947
Protein NameEphrin-B1
Description of TargetBinds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
Uniprot IDP98172
Protein Accession #NP_004420.1
Nucleotide Accession #NM_004429.4
Protein Size (# AA)346
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