Catalog No: OKCD01990 (Formerly GWB-KBBAY3)
Size:96WELLS
Price: $725.00
SKU
OKCD01990
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Datasheets/ManualsPrintable datasheet for DGAT1 ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)93-101%96-103%81-101%90-99%
EDTA Plasma (n=5)87-97%78-96%79-92%96-104%
Heparin Plasma (n=5)81-103%88-95%82-91%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)93-10196
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)88-9791
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Dgat1 Microplate96 Wells (12 x 8 Well Strips)
Dgat1 Lyophilized Standard2 x 50 ng
100X Biotinylated Dgat1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.25 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDGAT1
Gene Full NameDiacylglycerol O-acyltransferase 1
Alias SymbolsARAT, Dgat
NCBI Gene Id84497
Protein NameDiacylglycerol O-acyltransferase 1
Description of TargetCatalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. In liver, plays a role in esterifying exogenous fatty acids to glycerol.
Uniprot IDQ9ERM3
Protein Accession #NP_445889.1
Nucleotide Accession #NM_053437.1
Protein Size (# AA)498
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