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Catalog No: OKCD02047 (Formerly GWB-KBBEO8)
Size:96WELLS
Price: $750.00
SKU
OKCD02047
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Datasheets/ManualsPrintable datasheet for DSE ELISA kit (Human)
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dermatan Sulfate Epimerase (DSE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dermatan Sulfate Epimerase (DSE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DSE Microplate96 Wells (12 x 8 Well Strips)
DSE Lyophilized Standard2 x 20 ng
100X Biotinylated DSE Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.114 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dermatan Sulfate Epimerase (DSE). No significant cross-reactivity or interference between Dermatan Sulfate Epimerase (DSE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDSE
Gene Full Namedermatan sulfate epimerase
Alias Symbolschondroitin-glucuronate 5-epimerase;dermatan-sulfate epimerase;DS epimerase;DSEP;DSEPI;DS-epi1;EDSMC2;SART2;SART-2;squamous cell carcinoma antigen recognized by T-cells 2.
NCBI Gene Id29940
Protein NameQ9UL01
Description of TargetDermatan-sulfate epimerase
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UL01
Protein Accession #NP_001074445.1
Nucleotide Accession #NM_001080976.2
Protein Size (# AA)958
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