DOT1L ELISA Kit (Human) (OKCD02046)

Catalog No: OKCD02046 (Formerly GWB-KBBNV5)

Size:96WELLS

Human DOT1L ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-104%	92-105%	90-102%	80-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase (DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase (DOT1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	79-93	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DOT1L Microplate: 96 Wells (12 x 8 Well Strips)
  • DOT1L Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated DOT1L Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of DOT1 Like, Histone H3 Methyltransferase (DOT1L). No significant cross-reactivity or interference between DOT1 Like, Histone H3 Methyltransferase (DOT1L) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DOT1L
• Gene Full Name: DOT1 like histone lysine methyltransferase
• Alias Symbols: DOT1;DOT1 like histone H3K79 methyltransferase;DOT1-like histone methyltransferase;DOT1-like protein;DOT1-like, histone H3 methyltransferase;H3-K79-HMTase;histone H3-K79 methyltransferase;histone methyltransferase DOT1L;histone-lysine N-methyltransferase, H3 lysine-79 specific;KMT4;lysine N-methyltransferase 4.
• NCBI Gene Id: 84444
• Protein Name: Histone-lysine N-methyltransferase, H3 lysine-79 specific
• Description of Target: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
• Uniprot ID: Q8TEK3
• Protein Accession #: NP_115871.1
• Nucleotide Accession #: NM_032482.2
• Protein Size (# AA): 1739