DLC1 ELISA Kit (Human) (OKCD01918)

Catalog No: OKCD01918 (Formerly GWB-KBBOA4)

Size:96WELLS

Human DLC1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deleted In Liver Cancer 1 (DLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deleted In Liver Cancer 1 (DLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deleted In Liver Cancer 1 (DLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Deleted In Liver Cancer 1 (DLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deleted In Liver Cancer 1 (DLC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deleted In Liver Cancer 1 (DLC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DLC1 Microplate: 96 Wells (12 x 8 Well Strips)
  • DLC1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated DLC1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Deleted In Liver Cancer 1 (DLC1). No significant cross-reactivity or interference between Deleted In Liver Cancer 1 (DLC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DLC1
• Gene Full Name: DLC1 Rho GTPase activating protein
• Alias Symbols: HP, ARHGAP7, STARD12, p122-RhoGAP
• NCBI Gene Id: 10395
• Protein Name: Rho GTPase-activating protein 7
• Description of Target: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.
• Uniprot ID: Q96QB1
• Protein Accession #: NP_001157743.1
• Nucleotide Accession #: NM_001164271.1
• Protein Size (# AA): 1528