DGAT2 ELISA Kit (Human) (OKCD01987)

Catalog No: OKCD01987 (Formerly GWB-KBBOZ8)

Size:96WELLS

Human DGAT2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DGAT2 Microplate: 96 Wells (12 x 8 Well Strips)
  • DGAT2 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated DGAT2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 1.33 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DGAT2
• Gene Full Name: diacylglycerol O-acyltransferase 2
• Alias Symbols: acyl-CoA retinol O-fatty-acyltransferase;ARAT;diacylglycerol O-acyltransferase 2;diacylglycerol O-acyltransferase homolog 2;diacylglycerol O-acyltransferase-like protein 2;diglyceride acyltransferase 2;GS1999FULL;HMFN1045.
• NCBI Gene Id: 84649
• Protein Name: Diacylglycerol O-acyltransferase 2
• Description of Target: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT).
• Uniprot ID: Q96PD7
• Protein Accession #: NP_001240820.1
• Nucleotide Accession #: NM_001253891.1
• Protein Size (# AA): 388