DEK ELISA Kit (Human) (OKCA00772)

Catalog No: OKCA00772

Size:96 Wells

Human DEK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	97	92-101
  1:2	106	101-113
  1:4	93	89-98
  1:8	90	85-94

• ELISA Kit Principle: Aviva Systems Biology DEK ELISA Kit (Human) (OKCA00772) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEK has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DEK captured in the well.
• ELISA Kit Range: 23.44 pg/mL-1,500 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	90-100
  EDTA Plasma (n=4)	88	84-92

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level DEK were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level DEK were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • DEK Microplate: 96 Wells (12 x 8 Well strips)
  • DEK Lyophilized Standard: 2
  • 100X Biotinylated DEK Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 5.86 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DEK
• Gene Full Name: DEK proto-oncogene
• Alias Symbols: D6S231E
• NCBI Gene Id: 7913
• Protein Name: Protein DEK
• Description of Target: Involved in chromatin organization.
• Uniprot ID: P35659
• Protein Accession #: NP_001128181.1
• Nucleotide Accession #: NM_001134709.1