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Catalog No: OKCD02105 (Formerly GWB-KBBQN4)
Size:96WELLS
Price: $750.00
SKU
OKCD02105
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Datasheets/ManualsPrintable datasheet for DEFB112 ELISA Kit (Human) (OKCD02105)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)98-105%84-99%97-105%84-93%
EDTA Plasma (n=5)88-102%87-96%85-103%90-98%
Heparin Plasma (n=5)85-105%80-98%93-105%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 112 (DEFb112). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 112 (DEFb112). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 112 (DEFb112), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Beta 112 (DEFb112) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-9894
EDTA Plasma (n=5)79-9889
Heparin Plasma (n=5)90-10595
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 112 (DEFb112) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 112 (DEFb112) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DEFB112 Microplate96 Wells (12 x 8 Well Strips)
DEFB112 Lyophilized Standard2 x 5 ng
100X Biotinylated DEFB112 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 29.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Beta 112 (DEFb112). No significant cross-reactivity or interference between Defensin Beta 112 (DEFb112) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDEFB112
Gene Full Namedefensin beta 112
Alias Symbolsbeta-defensin 112;beta-defensin 12;DEFB-12;Defensin, beta 112;defensin, beta 12.
NCBI Gene Id245915
Protein NameBeta-defensin 112
Description of TargetHas antibacterial activity.
Uniprot IDQ30KQ8
Protein Accession #NP_001032587.1
Nucleotide Accession #NM_001037498.1
Protein Size (# AA)113
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