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Catalog No: OKCD01605 (Formerly GWB-KBBAY5)
Size:96WELLS
Price: $700.00
SKU
OKCD01605
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Datasheets/ManualsPrintable datasheet for CYSLTR2 ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 2 (CYSLTR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteinyl Leukotriene Receptor 2 (CYSLTR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cysltr2 Microplate96 Wells (12 x 8 Well Strips)
Cysltr2 Lyophilized Standard2 x 20 ng
100X Biotinylated Cysltr2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cysteinyl Leukotriene Receptor 2 (CYSLTR2). No significant cross-reactivity or interference between Cysteinyl Leukotriene Receptor 2 (CYSLTR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYSLTR2
Gene Full NameCysteinyl leukotriene receptor 2
Alias SymbolsCyslt2, RSBPT32
NCBI Gene Id170926
Protein NameCysteinyl leukotriene receptor 2
Description of TargetReceptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Uniprot IDQ924T9
Protein Accession #NP_596904.1
Nucleotide Accession #NM_133413.1
Protein Size (# AA)309
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