Catalog No: OKCD02044 (Formerly GWB-KBBMO9)
Size:96WELLS
Price: $750.00
SKU
OKCD02044
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Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 7, Member A (CLEC7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 7, Member A (CLEC7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Clec7a Microplate96 Wells (12 x 8 Well Strips)
Clec7a Lyophilized Standard2 x 10 ng
100X Biotinylated Clec7a Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 7, Member A (CLEC7A). No significant cross-reactivity or interference between C-Type Lectin Domain Family 7, Member A (CLEC7A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCLEC7A
Gene Full NameC-type lectin domain family 7, member a
Alias SymbolsBeta-glucan receptor;C-type lectin superfamily member 12;Dendritic cell-associated C-type lectin 1.
NCBI Gene Id56644
Protein NameC-type lectin domain family 7 member A
Description of TargetLectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
Uniprot IDQ6QLQ4
Protein Accession #NP_001296566.1
Nucleotide Accession #NM_001309637.1
Protein Size (# AA)244
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