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Catalog No: OKCD01744 (Formerly GWB-KBBTS4)
Size:96WELLS
Price: $700.00
SKU
OKCD01744
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Datasheets/ManualsPrintable datasheet for CHD4 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromodomain Helicase DNA Binding Protein 4 (CHD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromodomain Helicase DNA Binding Protein 4 (CHD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromodomain Helicase DNA Binding Protein 4 (CHD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromodomain Helicase DNA Binding Protein 4 (CHD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromodomain Helicase DNA Binding Protein 4 (CHD4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromodomain Helicase DNA Binding Protein 4 (CHD4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Chd4 Microplate96 Wells (12 x 8 Well Strips)
Chd4 Lyophilized Standard2 x 10 ng
100X Biotinylated Chd4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chromodomain Helicase DNA Binding Protein 4 (CHD4). No significant cross-reactivity or interference between Chromodomain Helicase DNA Binding Protein 4 (CHD4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCHD4
Gene Full NameChromodomain helicase DNA binding protein 4
Alias SymbolsMi-2be, AA617397, BC005710, Mi-2beta, D6Ertd380, mKIAA4075, D6Ertd380e, 9530019N15Rik
NCBI Gene Id107932
Protein NameChromodomain-helicase-DNA-binding protein 4
Description of TargetComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Uniprot IDQ6PDQ2
Protein Accession #NP_666091.1
Nucleotide Accession #NM_145979.2
Protein Size (# AA)1915
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