Catalog No: OKCD01914 (Formerly GWB-KBBAR5)
Size:96WELLS
Price: $725.00
SKU
OKCD01914
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Datasheets/ManualsPrintable datasheet for CELSR2 ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Celsr2 Microplate96 Wells (12 x 8 Well Strips)
Celsr2 Lyophilized Standard2 x 50 ng
100X Biotinylated Celsr2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.24 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2). No significant cross-reactivity or interference between Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCELSR2
Gene Full NameCadherin, EGF LAG seven-pass G-type receptor 2
Alias SymbolsMegf3
NCBI Gene Id83465
Protein NameCadherin EGF LAG seven-pass G-type receptor 2
Description of TargetReceptor that may have an important role in cell/cell signaling during nervous system formation.
Uniprot IDQ9QYP2
Protein Accession #XP_006233254.1
Nucleotide Accession #XM_006233192.3
Protein Size (# AA)2144
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